1. Home
  2. Vol 29, No 4 (2025)
  3. Liu

Paraneoplastic Ma antigen 5 promotes cellular processes of primary cells in ovarian endometriosis

Yinglei Liu(1), Zhe Chen(2), Jun You(3), Yanli Zheng(4), Haifeng Qiao(5),


(1) Department of Gynecology, The Second Affiliated Hospital of Nantong University, Nantong, 226001. China
(2) Medical College of Nantong University, Nantong, 226001. China
(3) Department of Gynecology, The Second Affiliated Hospital of Nantong University, Nantong, 226001. China
(4) Department of Gynecology, The Second Affiliated Hospital of Nantong University, Nantong, 226001. China
(5) Department of Gynecology, The Second Affiliated Hospital of Nantong University, Nantong, 226001. China
Corresponding Author

Abstract


This study investigates the role of paraneoplastic Ma antigen 5 (PNMA 5), a member of the PNMA family of proteins, in ovarian endometriosis (EM). These proteins are known to play a significant role in various physiological activities and are highly expressed in cells undergoing carcinogenesis, where they influence cell cycle, apoptosis, and other cellular processes. However, the involvement of PNMA 5 in ovarian EM has not been fully understood. To explore this, the study analyzed PNMA 5 levels in both normal and ectopic endometrial tissues from ovarian EM patients using immunohistochemistry. Small interfering RNA (siRNA) was used to knock down PNMA 5 in ectopic tissue cells, and the effects of this intervention were assessed through qRT-PCR and western blot to evaluate the efficiency of knockdown. Additionally, CCK-8 assays, flow cytometry, transwell, and wound healing assays were employed to examine the impact of PNMA 5 on cell proliferation, apoptosis, migration, and invasion. The results demonstrated that PNMA 5 expression was significantly higher in ectopic tissues compared to normal endometrium. Moreover, the protein inhibited cell proliferation and invasion while promoting apoptosis in primary cells from ectopic tissues. These findings suggest that PNMA 5 could serve as a potential therapeutic target for EM, though further research is needed to fully understand its mechanisms.

References


Giudice LC and Kao LC .Endometriosis. Lancet, 2004.

(9447): p. 1789-99.

Vercellini P, Vigano P, Somigliana E and Fedele L.

Endometriosis: pathogenesis and treatment. Nat Rev

Endocrinol, 2014. 10(5): p. 261-75.

Bulun SE, Yilmaz BD, Sison C, Miyazaki K, Bernardi L, Liu

S, Kohlmeier A, Yin P, Milad M and Wei J.

Endometriosis. Endocr Rev, 2019. 40(4): p. 1048

Mehedintu C, Plotogea MN, Ionescu S and Antonovici M.

Endometriosis still a challenge. J Med Life, 2014.

(3): p. 349-57.

Garcia-Fernandez J and Garcia-Velasco JA. Endometriosis

and Reproduction: What We Have Learned. Yale J

Biol Med, 2020. 93(4): p. 571-577.

Donnez J, Garcia-Solares J and Dolmans MM. Ovarian

endometriosis and fertility preservation: a challenge

in 2018. Minerva Ginecol, 2018. 70(4): p. 408-414.

Nowak-Psiorz I, Ciecwiez SM, Brodowska A and

Starczewski A Treatment of ovarian endometrial

cysts in the context of recurrence and fertility. Adv

Clin Exp Med, 2019. 28(3): p. 407-413.

Zhang L, Yu Z, Qu Q, Li X, Lu X and Zhang H. Exosomal

lncRNA HOTAIR Promotes the Progression and

Angiogenesis of Endometriosis via the miR

/HDAC1 Axis and Activation of STAT3

Mediated Inflammation. Int J Nanomedicine, 2022.

: p. 1155-1170.

Matta K, Koual M, Ploteau S, Coumoul X, Audouze K, Le

Bizec B, Antignac JP and Cano-Sancho G.

Associations between Exposure to Organochlorine

Chemicals and Endometriosis: A Systematic Review

of Experimental Studies and Integration of

Epidemiological Evidence. Environ Health Perspect,

129(7): p. 76003.

Ulukus, M. and A. Arici, Immunology of endometriosis.

Minerva Ginecol, 2005. 57(3): p. 237-48.

Barcz E, Kaminski P and Marianowski L, Role of cytokines

in pathogenesis of endometriosis. Med Sci Monit,

6(5): p. 1042-6.

Jensen JR and Coddington CC. 3rd, Evolving spectrum: the

pathogenesis of endometriosis. Clin Obstet Gynecol,

53(2): p. 379-88.

Nisolle M, Casanas-Roux F and Donnez J. Early-stage

endometriosis: adhesion and growth of human

menstrual endometrium in nude mice. Fertil Steril,

74(2): p. 306-12.

Bazot M and Darai E. Diagnosis of deep endometriosis:

clinical examination, ultrasonography, magnetic

resonance imaging, and other techniques. Fertil

Steril, 2017. 108(6): p. 886-894.

Mate G, Bernstein LR and Torok AL. Endometriosis Is a

Cause of Infertility. Does Reactive Oxygen Damage

to Gametes and Embryos Play a Key Role in the

Pathogenesis of Infertility Caused by Endometriosis?

Front Endocrinol (Lausanne), 2018. 9: p. 725.

Vinatier D, Bernardi C, Maunoury-Lefebvre C, Dufour P,

Depret-Mosser S, Tiberghien B and Monnier JC.

[Immunologic aspects of endometriosis]. J Gynecol

Obstet Biol Reprod (Paris), 1992. 21(8): p. 877-86.

Suginami H, A reappraisal of the coelomic metaplasia

theory by reviewing endometriosis occurring in

unusual sites and instances. Am J Obstet Gynecol,

165(1): p. 214-8.

van der Linden PJ. Endometriosis: a review on its

pathogenesis. Front Biosci, 1997. 2: p. e48-52.

Gil Y and Tulandi T. Diagnosis and Treatment of

Catamenial Pneumothorax: A Systematic Review. J

Minim Invasive Gynecol, 2020. 27(1): p. 48-53.

Zakhari A, Edwards D, Ryu M, Matelski JJ, Bougie O and

Murji A, Dienogest and the Risk of Endometriosis

Recurrence Following Surgery: A Systematic

Review and Meta-analysis. J Minim Invasive

Gynecol, 2020. 27(7): p. 1503-1510.

Hill WG, Why aren't horses faster? Nature, 1988.

(6166): p. 678.

Won S, Cho YJ, Lee N, Kim M, Kim MK, Jung YW, Yun

BS, Seong SJ, Hur J and Kim ML. Atypical

endometriosis is related to a higher recurrence rate.

Eur J Obstet Gynecol Reprod Biol, 2020. 254: p. 44

Pang SW, Lahiri C, Poh CL and Tan KO. PNMA family:

Protein interaction network and cell signalling

pathways implicated in cancer and apoptosis. Cell

Signal, 2018. 45: p. 54-62.

Zhou Y, Zang Y, Yang Y, Xiang J and Chen Z. Candidate

genes involved in metastasis of colon cancer

identified by integrated analysis. Cancer Med, 2019.

(5): p. 2338-2347.

Lee YH, Pang SW, Poh CL and Tan KO. Distinct functional

domains of PNMA5 mediate protein-protein

interaction, nuclear localization, and apoptosis

signaling in human cancer cells. J Cancer Res Clin

Oncol, 2016. 142(9): p. 1967-77.

Dong XY, Yang X, Su Y, Li Y, Qiao H, Yin Y and Chen

F. Cloning and Identification of PNMA5，a Novel

Member of Cancer-Testis--Brain Antigen Family.

Chinese Journal of Biochemistry and Molecular

Biology, 2004(05): p. 587-591.

Teng XM, Deng Q, Han ZG and Huang J. Expression of

PNMA5 in hepatocellular carcinoma tissues and its

function. Chinese Journal, Tumor, 2008(11): p. 911

Takaji M, Komatsu Y, Watakabe A, Hashikawa T and

Yamamori T. Paraneoplastic antigen-like 5 gene

(PNMA5) is preferentially expressed in the

association areas in a primate specific manner. Cereb

Cortex, 2009. 19(12): p. 2865-79.

Zhang XL, Liu P, Yang ZX, Zhao JJ, Gao LL, Yuan B, Shi

LY, Zhou CX, Qiao HF, Liu YH, Ying XY, Zhang

JQ, Ling XF and Zhang D. Pnma5 is essential to the

progression of meiosis in mouse oocytes through a

chain of phosphorylation. Oncotarget, 2017. 8(57): p.

-96825.

Qiao H, Liu J, Yang Z, Gao L, Zhang X, Lu G, Liu Y, Liu

M, Ying X and Zhang D. PNMA5 knockdown

suppresses the proliferation, invasion, and metastasis

of epithelial ovarian cancer cells. Transl Cancer Res,

9(5): p. 3691-3702.

Bulun SE, Wan Y and Matei D. Epithelial Mutations in

Endometriosis:

Link

to

Ovarian

Cancer.

Endocrinology, 2019. 160(3): p. 626-638.

Murakami K, Kotani Y, Shiro R, Takaya H, Nakai H and

Matsumura N. Endometriosis-associated ovarian

cancer occurs early during follow-up of endometrial

cysts. Int J Clin Oncol, 2020. 25(1): p. 51-58.

Guidozzi F. Endometriosis-associated cancer. Climacteric,

24(6): p. 587-592.

Mikhaleva LM, Davydov AI, Patsap OI, Mikhaylenko EV,

Nikolenko VN, Neganova ME, Klochkov SG,

Somasundaram SG, Kirkland CE and Aliev G.

Malignant

Transformation

and

Associated

Biomarkers of Ovarian Endometriosis: A Narrative

Review. Adv Ther, 2020. 37(6): p. 2580-2603.

Gadducci A Lan, fredini N and Tana R. Novel insights on

the malignant transformation of endometriosis into

ovarian carcinoma. Gynecol Endocrinol, 2014.

(9): p. 612-7.

Huang F, Cao Y, Wang C, Lan R, Wu B, Xie X, Hong J,

Fu L and Wu G. PNMA5 Promotes Bone Metastasis

of Non-small-Cell Lung Cancer as a Target of BMP2

Signaling. Front Cell Dev Biol, 2021. 9: p. 678931.

Lin J, Zhang X, Meng F, Zeng F, Liu W and He X. PNMA5

accelerated cellular proliferation, invasion and

migration in colorectal cancer. Am J Transl Res,

14(4): p. 2231-2243.


Full Text: PDF

Article Metrics

Abstract View : 617 times
PDF Download : 239 times

Refbacks

  • There are currently no refbacks.